C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) C12N 9/80 (2006.01) C12N 15/10 (2006.01) C12P 13/04 (2006.01) C12P 41/00 (2006.01)
Patent
CA 2454701
The invention relates to a nucleic acid sequence encoding an enantioselective amidase with an amino acid sequence, which amino acid sequence has at least 70% identity with SEQ ID NO: 2. The invention also relates to a process for the fermentation, comprising a batch and a feed phase, of a microorganism in a fermentation medium, wherein the microorganism expresses a nucleic acid according to the invention and wherein between 0.5 and 50mg/l_fermentation medium Zn2+ is fed during the fermentation. The invention also relates to a process for the preparation of an enantiomerically enriched carboxylic acid and/or an enantiomerically enriched carboxylic acid amide, in which a mixture of the corresponding D- and L-carboxylic acid amides is contacted with an expression product according to the invention in the presence of between 0.01mM and 100mM Zn2+, whereby one of the enantiomers of the carboxylic acid amide is enantioselectively hydrolysed to form the corresponding enantiomerically enriched carboxylic acid, while the other enantiomer of the carboxylic acid amide remains unchanged.
L'invention concerne une séquence d'acides nucléiques codant pour une amidase énantiosélective, ladite séquence étant au moins à 70% identique à SEQ ID NO: 2. L'invention concerne également un procédé de fermentation ,comprenant une phase de lot et d'alimentation, d'un micro-organisme dans un milieu de fermentation, ledit micro-organisme exprimant un acide nucléique selon ladite invention, 0,5 à 50mg/l du milieu de fermentation Zn?2+¿ étant fourni pendant ladite fermentation. L'invention concerne enfin un procédé de préparation d'un acide carboxylique et/ou d'un amide d'acide carboxylique enrichi(s) en énantiomères, dans lequel un mélange d'acides D-carboxylique et L-carboxylique correspondant est mis en contact avec un produit d'expression selon l'invention en présence de 0,01mM à 100mM de Zn?2+¿, l'un des énantiomères de l'amide d'acide carboxylique étant hydrolysé de manière énantiosélective afin de former un acide carboxylique correspondant enrichi en énantiomères, l'autre énantiomère de l'amide d'acide carboxylique restant inchangé.
Assema Van Friso Bernard Jan
Korevaar Cornelis Gerrit Nijs
Pol Van Der Rudolf
Sonke Theodorus
Tandler Renate Francisca
Dsm Ip Assets B.v.
Fetherstonhaugh & Co.
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