Nucleic acid sequences encoding proteins capable of...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01) A61K 39/15 (2006.01) A61K 39/395 (2006.01) C07K 14/14 (2006.01) C07K 14/165 (2006.01) C07K 14/17 (2006.01)

Patent

CA 2591532

The present invention relates to nucleic acids comprising: (a) sequences of a replication competent transmissible gastroenteritis virus (TGEV), which sequences encode a TGEV replicase under the control of expression regulatory sequences so that expression of the replicase in a cell containing the nucleic acid will initiate replication of the nucleic acid and thus increase the number of nucleic acids in the cell; and (b) sequences encoding one or more proteins of a different virus wherein the one or more proteins are capable of associating into a virus-like particle (VLP) that does not contain any infectious nucleic acid. The present invention further relates to vectors, virus particles and host cells comprising these nucleic acids as well as their use for the preparation of vaccines, specifically for the preparation of vaccines.

La présente invention concerne des acides nucléiques comprenant (a) des séquences d'un virus réplicatif responsable des gastro-entérites transmissibles (TGEV), lesquelles séquences codent pour une réplicase du TGEV sous le contrôle de séquences régulatrices d'expression, de sorte que l'expression de la réplicase dans une cellule contenant l'acide nucléique va initier la réplication de l'acide nucléique et augmenter ainsi le nombre d'acides nucléiques dans la cellule, et (b) des séquences codant pour une ou plusieurs protéines d'un virus différent, lesdites une ou plusieurs protéines étant capables de s'associer à une pseudo-particule virale (VLP) ne contenant pas d'acide nucléique infectieux. Cette invention concerne également des vecteurs, particules virales et cellules hôtes comprenant ces acides nucléiques ainsi que leur utilisation dans la préparation de vaccins, spécifiquement dans la préparation de vaccins.

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