Nucleic acid sequencing system and method

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) G06T 7/00 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2676570

A technique for sequencing nucleic acids in an automated or semi-automated manner is disclosed. Sample arrays of a multitude of nucleic acid sites are processed in multiple cycles to add nucleotides to the material to be sequenced, detect the nucleotides added to sites, and to de-block the added nucleotides of blocking agents and tags used to identify the last added nucleotide. Multiple parameters of the system are monitored to enable diagnosis and correction of problems as they occur during sequencing of the samples. Quality control routines are run during sequencing to determine quality of samples, and quality of the data collected.

L'invention concerne un procédé destiné à séquencer des acides nucléiques de manière automatisée ou semi-automatisée. Des réseaux d'échantillons présentant une multitude de sites d'acides nucléiques sont traités dans plusieurs cycles pour ajouter des nucléotides à la matière devant subir un séquençage, afin de détecter les nucléotides ajoutés à ces sites et de débloquer les nucléotides ajoutés de marquages et d'agents bloquants utilisés pour identifier le nucléotide ajouté en dernier. Plusieurs paramètres du système sont surveillés pour établir un diagnostic et corriger les problèmes, dès leur apparition, pendant le séquençage des échantillons. Des routines de contrôle de qualité sont effectuées pendant le séquençage pour déterminer la qualité des échantillons et la qualité des données recueillies.

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