C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
195/1.23, 150/8.
C12Q 1/68 (2006.01) G01N 33/58 (2006.01) A61K 49/02 (1980.01)
Patent
CA 1179928
ABSTRACT OF THE DISCLOSURE A method of detecting a specific DNA sequence in eucaryotic cells or a biological field using nucleic acid spot hybridization comprising the steps of contacting eucaryotic cells or a biological fluid with a support material capable of binding DNA, lysing the cells (when present) and denaturing the DNA so that the DNA present is immobilized on the support material, treating the support-material-immobilized DNA with a detectably-tagged DNA or RNA probe that is capable of forming a double- stranded structure with the specific DNA sequence, and detecting the tagged probe to determine the presence of double-stranded structure, which indicates the presence of the initially sought specific DNA sequence. Preferred support material is nitrocellulose, to which cells, single-stranded, and double-stranded DNA all adhere, but to which double-stranded DNA adheres most strongly.
394758
Brandsma Janet
Miller George
Research Corporation
Swabey Ogilvy Renault
LandOfFree
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