Nucleic acid synthesis using photoremovable protecting groups

Details Nucleic acid synthesis using photoremovable protecting groups Nucleic acid synthesis using photoremovable protecting groups

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) B01J 19/08 (2006.01) C07H 1/00 (2006.01) C07H 19/10 (2006.01) C07H 19/20 (2006.01) C07H 23/00 (2006.01)

Patent

CA 2215657

The present invention relates, in general, to a method of synthesizing a nucleic acid, and, in particular, to a method of effecting 5' to 3' nucleic acid synthesis. The method can be used to prepare arrays of oligomers bound to a support via their 5' end. The invention also relates to a method of effecting mutation analysis using such arrays. The invention further relates to compounds and compositions suitable for use in such methods.

La présente invention concerne, de manière générale, un procédé de synthèse d'un acide nucléique et, en particulier, un procédé permettant d'effectuer la synthèse d'acide nucléique de 5' à 3' . Ce procédé peut être utilisé dans la préparation de réseaux d'oligomères liés à un support par leur extrémité 5'. Cette invention concerne également un procédé permettant d'effectuer une analyse de mutation à l'aide de tels réseaux. Cette invention concerne enfin des composés et des compositions pouvant être utilisés dans de tels procédés.

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