Nucleic acid transfer system

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/87 (2006.01) A61K 31/70 (2006.01) A61K 47/48 (2006.01) A61K 48/00 (2006.01) C07K 14/34 (2006.01) C07K 19/00 (2006.01) C12N 15/31 (2006.01) C12N 15/62 (2006.01)

Patent

CA 2204020

The invention pertains to a nucleic acid transfer system including a translocation domain of toxins, especially of diphtheria toxin suitable for targeting a nucleic acid, e.g. a gene, to a specific cell, and obtaining expression of said nucleic acid. The nucleic acid transfer system of the invention comprises a multidomain protein component and a nucleic acid component. Furthermore, the present invention relates to the multidomain protein, a nucleic acid encoding said protein, suitable amplification and expression systems for said nucleic acid, and processes for the preparation and uses of the above subject matters.

L'invention concerne un système de transfert de l'acide nucléique comprenant un domaine de translocation de toxines, en particulier la toxine de la diphtérie appropriée pour le ciblage d'un acide nucléique, p. ex. un gène, sur une cellule spécifique, et l'obtention de l'expression dudit acide nucléique. Le système de transfert de l'acide nucléique de l'invention comprend un composant protéine multi-domaine et un composant acide nucléique. En outre, la présente invention concerne la protéine multi-domaine, un acide nucléique codant ladite protéine, des systèmes appropriés d'amplification et d'expression pour ledit acide nucléique, et des procédés de préparation et utilisation des matières mentionnées ci-dessus.

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