Nucleic acid vaccine compositions having a mammalian...

A - Human Necessities – 61 – K

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A61K 39/00 (2006.01) A61K 39/21 (2006.01) A61K 39/29 (2006.01) A61K 48/00 (2006.01) C07K 14/475 (2006.01) C07K 14/705 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2389680

Polynucleotides encoding at least one immunizing antigen whose expression is controlled by a promoter derived from a gene encoding a co-stimulatory molecule are provided. The polynucleotides may also encode adjuvants. Compositions comprising at least one immunizing agent and at least one cytokine that enhance dendritic cell stimulation and/or survival are also provided. Methods for eliciting an immune response against the immunizing agent are also provided. The method includes the steps of administering the polynucleotides and, optionally, co-administering an adjuvant.

La présente invention concerne des polynucléotides codant au moins un antigène immunisant dont l'expression est commandée par un promoteur dérivé d'un gène codant une molécule de co-stimulation. Ces polynucléotides peuvent également coder des adjuvants. L'invention concerne également des compositions comprenant au moins un agent immunisant et au moins une cytokine renforçant la stimulation et/ou la survie des cellules dendritiques. L'invention concerne enfin des procédés permettant de mettre en évidence une réponse immunitaire contre l'agent immunisant. Un tel procédé fait intervenir l'administration de ces polynucléotides, et éventuellement, la co-administration d'un adjuvant.

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