Nucleic acids and libraries

Details Nucleic acids and libraries Nucleic acids and libraries

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12N 5/10 (2006.01) C12N 15/52 (2006.01) C12N 15/62 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2684792

The invention relates to a nucleic acid comprising the following contiguous elements arranged in the 5 prime to 3 prime direction; a promoter; a selectable marker; a cloning site for receipt of a nucleic acid segment, said segment comprising a candidate miRNA target sequence; and a poly adenylation signal, said elements arranged such that a transcript directed by said promoter comprises said selectable marker, said candidate miRNA target sequence, and said poly adenylation signal in that order. Suitably the miRNA test sequence is or is derived from a 3'UTR. The invention also relates to methods for making and screeing libraries.

L'invention concerne un acide nucléique comprenant les éléments contigus suivants agencés dans la direction 5 prime vers 3 prime; un promoteur; un marqueur sélectionnable; un site de clonage pour recevoir un segment d'acide nucléique, ledit segment comprenant une séquence cible d'ARNmi candidat; et un signal de polyadénylation, lesdits éléments étant agencés de telle sorte qu'un transcrit dirigé par ledit promoteur comprend ledit marqueur sélectionnable, ladite séquence cible d'ARNmi candidat, et ledit signal de polyadénylation, dans cet ordre. De préférence, la séquence à tester d'ARNmi est une 3'UTR ou est dérivée d'une 3'UTR. L'invention concerne également des procédés pour préparer et cribler des bibliothèques.

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