Nucleic acids and methods for detecting turfgrass pathogenic...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2726699

The present invention relates to the use of at least one nucleic acid comprising or consisting of: (i) CATCGAT- GAAGAACGCWGCRAAHTGCGATAMGTARTGYGAATTGCAGRATTCAGTGARTCATCGAAWYTTTGAACGCAYMTTGCRC (SEQ ID NO: 1), wherein: R represents A or G Y represents C or T M represents A or C W represents A or T H represents A or C or T (ii) a portion of SEQ ID NO: 1, provided said nucleic acid binds under stringent conditions to a nucleic acid comprising or consisting of the complementary sequence of SEQ ID NO: 1, or (iii) complementary sequences of (i) and (ii); for the detection of nucleic acids from one or more fungi in a sample.

La présente invention concerne lutilisation dau moins un acide nucléique comprenant ou constitué de : (i) CATCGATGAAGAACGCWGCRAAHTGCGATAMGTARTGYGAATTGCAGR ATTCAGTGARTCATCGAAWYTTTGAACGCAYMTTGCRC (SEQ ID NO : 1), dans laquelle : R représente A ou G, Y représente C ou T, M représente A ou C, W représente A ou T, H représente A ou C ou T (ii) une portion de SEQ ID NO : 1, à condition que ledit acide nucléique se lie dans des conditions stringentes à un acide nucléique comprenant ou constitué de la séquence complémentaire de SEQ ID NO : 1, ou (iii) de séquences complémentaires de (i) et (ii); pour la détection dacides nucléiques provenant dun ou plusieurs champignons dans un échantillon.

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