Nucleic acids for detecting aspergillus species and other...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12N 15/11 (2006.01)

Patent

CA 2288965

Nucleic acids for detecting Aspergillus species and other filamentous fungi are provided. Unique internal transcribed spacer 2 coding regions permit the development of nucleic acid probes specific for five different species of Aspergillus, three species of Fusarium, four species of Mucor, two species of Penecillium, five species of Rhizopus, one species of Rhizomucor, as well as probes for Absidia corymbifera, Cunninghamella elagans, Pseudallescheria boydii, and Sporothrix schenkii. The invention thereby provides methods for the species-specific detection and diagnosis of infection by Aspergillus, Fusarium, Mucor, Penecillium, Rhizopus, Rhizomucor, Absidia, Cunninghamella, Pseudallescheria or Sporthrix in a subject. Furthermore, genus- specific probes are also provided for Aspergillus, Fusarium and Mucor, in addition to an all-fungus nucleic acid probe.

L'invention concerne des acides nucléiques pour la détection du genre Aspergillus et autres champignons filamenteux. Des régions de codage par unique espaceur 2 interne transcrit permettent le développement de sondes à l'acide nucléique spécifiques pour cinq espèces différentes d'Aspergillus, trois espèces de Fusarium, quatre espèces de Mucor, deux espèces de Penecillium, cinq espèces de Rhizopus, une espèce de Rhizomucor, et aussi de sondes pour Absidia corymbifera, Cunninghamella elagans, Pseudallescheria boydii et Sporothrix schenkii. L'invention concerne en outre des procédés pour la détection propre aux espèces et le diagnostic des infections engendrées par Aspergillus, Fusarium, Mucor, Penecillium, Rhizopus, Rhizomucor, Absidia, Cunninghamella, Pseudallescheria ou Sporothrix chez un sujet. L'invention concerne également des sondes propres aux gènes pour Aspergillus, Fusarium et Mucor, venant s'ajouter à une sonde à l'acide nucléique tous champignons.

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