Nucleid acids and polypeptides encoded thereby

C - Chemistry – Metallurgy – 12 – N

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530/13, 167/139,

C12N 15/12 (2006.01) A61K 38/18 (2006.01) A61K 39/395 (2006.01) C07K 14/475 (2006.01) C07K 16/22 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2006744

ABSTRACT Amplified signals in both existing and non-detected restriction fragments were observed with 3 different enzymes (PVU2, HindIII and EcoRI) in two different AMLM2 cases out of 5. Most interestingly, the amplification of clone 14-hybridizable DNA was accompanied by amplification in BuChE positive sequences. The fact that different sizes of the restriction fragments hybridized with the 3' probe excluded the possibility of cross-hybridization, Several of the amplified bands could be observed, with lower intensities, in the presumptive polycytemia vera (Vaguez disease) case or in one or two of the other AML cases. The adr. sample represents a patient, currently under remission following chemotherapy treatment, whose serum and erythrocyte ChE were apparently normal in levels and biochemical characteristics. ChE genes and clone 14 are both co-regulated and co-amplified under specific stimulants.

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