Nucleoside analogue method

Details Nucleoside analogue method Nucleoside analogue method

C - Chemistry, Metallurgy

12

P

C12P 19/30 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2174942

A method of making a dideoxynucleoside mono- or tri-phosphate, which is optionally 32-P or 33-P or 35-5 radiolabelled, involves reacting the corresponding dideoxynucleoside with an optionally radiolabelled nucleotide phosphate or thiophosphate donor in the presence of a kinase or phosphotranferase enzyme which catalyses the reaction. A method of sequencing nucleic acids by a chain termination technique involves detecting the products of enzymatic synthesis by means of isotopically labelled chain terminating nucleotide analogues.

Un procédé de production d'un mono- ou triphosphate didésoxynucléosidique, qui est éventuellement radiomarqué 32-P ou 33-P ou 35-S, consiste à faire réagir le didésoxynucléoside correspondant avec un doneur de phosphate ou thiophosphate nucléotidique éventuellement radiomarqué en présence d'une kinase ou phosphotransférase qui catalyse la réaction. Un procédé de séquençage d'acides nucléiques, effectué à l'aide d'une technique de terminaison en chaîne, consiste à détecter les produits de synthèse enzymatique au moyen d'analogues nucléotidiques de terminaison de chaîne, marqués isotopiquement.

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