Nucleotide extension on a microarray of gel-immobilized primers

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2369524

Methods and compositions have been developed for nucleotide extension of primers immobilized within gel pads on a microchip using multibase primers or multiple sets of primers, or combinations thereof. Molecules or parts of molecules are identified. The single base extension was amplified by carrying out the reaction under elevated temperature.

L'invention concerne des méthodes et des compositions destinées à l'extension nucléotidique d'amorces immobilisées dans des matrices de gel sur une microplaquette utilisant des amorces définissant plusieurs bases ou des séries multiples d'amorces, ou des combinaisons de celles-ci. Des molécules ou des fractions de molécules sont identifiées. L'extension à base unique a été amplifiée par l'exécution de la réaction dans des conditions de hautes températures.

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