Nucleotide fragment, probe, primer, reagent and method for...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2366108

The invention concerns a single-strand nucleotide fragment comprising a sequence of at least twelve adjacent nucleotide motifs capable of being hybridised, in conditions of high stringency, with SEQ ID NO: 1 or it complementary sequence. The invention also concerns the uses of said fragment as probe, primer, in a reagent and in a method for detecting a nucleotide sequence derived from pBR322 replication origin in a biological sample.

L'invention concerne un fragment nucléotidique monocaténaire comprenant une séquence d'au moins douze motifs nucléotidiques contigus susceptibles de s'hybrider, dans des conditions de forte stringence, à SEQ ID NO :1 ou à son complémentaire. Elle concerne aussi les utilisations d'un tel fragment comme sonde, amorce, dans un réactif et dans un procédé de détection d'une séquence nucléotidique dérivée de l'origine de réplication de pBR322, dans un échantillon biologique.

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