C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/04 (2006.01)
Patent
CA 2257182
Nucleotide probe selected in particular among the following: TG <u>CGT TAT GTG ACC AGA</u>, GT<u>G CGT CTT GTG ACC AGA</u>, GT <u>CTT GTA ACC AGA CAC</u> AT, CG<u>T CTT GTA ACC AGA TAC</u> AT, CG<u>T CTT GTG AGC AGA AGC</u> AT, CG <u>ACC GAG CTC GTG CGG CG</u>T G, G T<u>AC CGG GCA GTG AC</u>G C, G A<u>CG CCG CTG GGI CCG CCT G</u>, G A<u>CG CCG CTG GGG CCG CCT G</u>, G G<u>AG GGI ACC CIG GCI GA</u>G T, G G<u>AG GGG ACC CGG GCG GA</u>G T, TC<u>G GTG GAC ACC GTA TGC AG</u>A C, GG <u>ACG GAG CGC GTG </u>CG, C <u>ATC TAT AAC CGA GA</u>, and their complementary sequences, being understood that the said probes have at least the underlined sequence, and can further contain one or two bases selected, respecting the sequence continuity, among the non-underlined bases. These probes are useful for determining a person's HLA DQ beta genotype. They have in particular the advantage of being useable at a single hybridization temperature, in particular 37 ·C.
Sonde nucléotidique choisie notamment parmi les suivantes: TG <u>CGT TAT GTG ACC AGA</u>; GT<u>G CGT CTT GTG ACC AGA</u>; GT <u>CTT GTA ACC AGA CAC</u> AT; CG<u>T CTT GTA ACC AGA TAC</u> AT; CG<u>T CTT GTG AGC AGA AGC</u> AT; GG <u>ACC GAG CTC GTG CGG GG</u>T G; G T<u>AC CGG GCA GTG AC</u>G C; G A<u>CG CCG CTG GGI CCG CCT G</u>; G A<u>CG CCG CTG GGG CCG CCT G</u>; G G<u>AG GGI ACC CIG GCI GA</u>G T; G G<u>AG GGG ACC CGG GCG GA</u>G T; TC<u>G GTG GAC ACC GTA TGC AG</u>A C; GG <u>ACG GAG CGC GTG</u> CG; C <u>ATC TAT AAC CGA GA</u>; et leurs complémentaires, étant entendu que lesdites sondes ont au minimum la séquence soulignée, et peuvent contenir en outre une ou deux bases choisies, en respectant la continuité de la séquence, parmi les bases non soulignées. Ces sondes sont utilisables pour déterminer le génotype HLA DQ bêta d'un individu. Elles présentent notamment l'avantage d'être utilisables à une température d'hybridation unique, en particulier 37 ~C.
Bio Merieux
Robic
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