Nucleotide sequence incoding plant ribosome inactivating...

C - Chemistry – Metallurgy – 12 – N

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195/1.22, 195/1.

C12N 15/29 (2006.01) A61K 38/16 (2006.01) A61K 47/48 (2006.01) C07K 14/415 (2006.01) C07K 19/00 (2006.01) C12N 15/62 (2006.01) A61K 38/00 (2006.01)

Patent

CA 1340805

A DNA sequence encoding the RIP SO-6 has the sequence: 1 24 50 GTCACATCAATCACATTAGATCTAGTAAATCCGACCGCGGGTCAATACTCATCTTTTGTG 100 GATAAAATCCGAAACAACGTAAAGGATCCAAACCTGAAATACGGTGGTACCGACATAGCC 150 GTGATAGGCCCACCTTCTAAAGAAAAATTCCTTAGAATTAATTTCCAAAGTTCCCGAGGA 200 ACGGTCTCACTTGGCCTAAAACGCGATAACTTGTATGTGGTCGCGTATCTTGCAATGGAT 250 300 AACACGAATGTTAATCGGGCATATTACTTCAGATCAGAAATTACTTCCGCCGAGTCAACC 350 GCCCTTTTCCCAGAGGCCACAACTGCAAATCAGAAAGCTTTAGAATACACAGAAGATTAT 400 CAGTCGATTGAAAAGAATGCCCAGATAACACAAGGAGATCAAAGTAGAAAAGAACTCGGG 450 TTGGGGATTGACTTACTTTCAACGTCCATGGAAGCAGTGAACAAGAAGGCACGTGTGGTT 500 AAAGACGAAGCTAGATTCCTTCTTATCGCTATTCAGATGACGGCTG 550 AGGCAGCGCGATTTAGGTACATACAAAACTTGGTAATCAAGAACTTTCCCAACAAGTTCAA 600 CTCGGAAAACAAAGTGATTCAGTTTGAGGTTAACTGGAAAAAAATTTCTACGGCAATATA 650 700 CGGGGATGCCAAAAACGGCGTGTTTAATAAAGATTATGATTTCGGGTTTGGAAAAGTTAG 750 GCAGGTGAAGGACTTGCAAATGGGACTCCTTATGTATTTGGGCAAACCAAAGTCGTC 768 AAAC. This sequence codes for a ribosomal inactivating protein (RIP) which can be used to inhibit protein synthesis by animal cells. Immunotoxins can be prepared including such an RIP.

603421

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