Nucleotide sequence of escherichia coli pathogenicity islands

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) C07K 14/245 (2006.01) C07K 16/12 (2006.01) C12N 15/63 (2006.01) G01N 33/569 (2006.01) G06F 17/30 (2006.01) G11B 7/00 (2006.01)

Patent

CA 2272047

The present invention relates to novel genes located in two chromosomal regions within uropathogenic E. coli that are associated with virulence. These chromosomal regions are known as pathogenicity islands (PAIs). In particular, the present application discloses 142 sequenced fragments (contigs) of DNA from two pools of cosmids covering pathogenicity islands PAI IV and PAI V located on the chromosome of the uropathogenic Escherichia coli J96. Further disclosed are 351 predicted protein-coding open reading frames within the sequenced fragments.

La présente invention se rapporte à de nouveaux gènes situés dans deux régions chromosomiques présentes à l'intérieur du bacille E. coli uropathogène et associées à la virulence de cet organisme pathogène. Ces régions chromosomiques sont connues comme étant des îlots de pathogénicité (PAI pathogenicity islands). L'invention se rapporte notamment à 142 fragments séquencés d'ADN (contigs) provenant de deux groupes de cosmides recouvrant les îlots de pathogénicité PAI IV et PAI V situés sur le chromosome de Escherichia coli J96 uropathogène. L'invention se rapporte en outre à 351 cadres ouverts de lecture codant les protéines prévues, présents à l'intérieur des fragments séquencés.

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