Nucleotide sequences for detecting enterohemorrhagic...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/08 (2006.01) C07H 21/00 (2006.01) C07K 14/245 (2006.01) C12N 15/31 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2326627

The invention concerns nucleic sequences of plasmid origin, present in bacteria of the group enterohemorrhagic Escherichia coli (EHEC), the use of said sequences for searching for EHEC, in particular those having genes coding for enterohemolysin and intimin virulence factors, and more particularly for specific detection of the O157:H7 serotype. The invention also concerns a method using said sequences and detection kits containing them.

La présente invention a pour objet des séquences nucléiques d'origine plasmidique, présentes chez les bactéries du groupe Escherichia coli entérohémorragiques (EHEC), l'utilisation desdites séquences pour la recherche des EHEC, notamment ceux possédant les gènes codant pour les facteurs de virulence entérohémolysine et intimine, et plus particulièrement la détection spécifique du sérotype O157:H7. L'invention vise également un procédé mettant en oeuvre lesdites séquences ainsi que les trousses de détection les contenant.

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