Nucleotide sequences for the tal gene

Details Nucleotide sequences for the tal gene Nucleotide sequences for the tal gene

C - Chemistry, Metallurgy

12

N

C12N 15/54 (2006.01) C12N 9/04 (2006.01) C12N 9/10 (2006.01) C12N 15/62 (2006.01) C12P 13/06 (2006.01) C12P 13/08 (2006.01) C12P 13/22 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2348448

The invention relates to an isolated polynucleotide from coryneform bacteria, comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequences of SEQ ID NO. 2 or SEQ ID NO. 4, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequences of SEQ ID NO. 2 or SEQ ID NO. 4, c) polynucleotide which is complementary to the polynucleotides of a) or b) and, d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequences of a), b) or c) and a process for the preparation of L-amino acids, which comprises carrying out the following steps: a) fermentation of the desired L-amino acid-producing bacteria in which at least the tal gene is amplified, b) concentration of the desired product in the medium or in the cells of the bacteria and c) isolation of the L-amino acid.

La présente invention concerne un polynucléotide isolé à partir d'une bactérie de type coryne, comportant une séquence polynucléotidique choisie dans le groupe constitué par: a) un polynucléotide identique jusqu'à au moins 70 % à un polynucléotide codant pour un polypeptide qui contient des séquences d'acide aminé de SEQ ID NO. 2 ou SEQ ID NO. 4, b) un polynucléotide codant pour un polypeptide qui contient une séquence d'acide aminé identique jusqu'à au moins 70 % aux séquences d'acide aminé de SEQ ID NO. 2 ou SEQ ID NO. 4, c) un polynucléotide complémentaire aux polynucléotides de a) ou b) et, d) un polynucléotide contenant au moins 15 nucléotides successifs des séquences polynucléotidiques de a), b) ou c) et un procédé de préparation de L-amino acides, consistant à: a) fermenter la bactérie voulue produisant le L-aminoacide où au moins le gène tal est amplifié, b) concentrer le produit voulu dans le milieu ou dans les cellules de la bactérie et c) isoler le L-aminoacide.

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