Nucleotide sequences hybridizing specifically with a genomic...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2203933

The present invention relates to a nucleic acid sequence isolated from C. coli which hybrizes specifically with the genomic nucleic acid of strains belonging to the species C. coli and which does not hybridize in usual conditions with the nucleic acids of campylobacteria which do not belong to said species, neither with the genomic nucleic acids of bacteria close to the genus Campylobacter. Fragments of said sequence may be used as specific primers for the amplification of specific sequences of Campylobacter coli and as nucleic probes specific to nucleic sequences of Campylobacter coli. The invention also relates to a method for detecting the presence of strains of Campylobacter coli in a biological sample as well as to a kit for implementing such method.

La présente invention a pour objet une séquence d'acide nucléique isolée de C. coli qui hybride spécifiquement avec l'acide nucléique génomique des souches appartenant à l'espèce C. coli et qui n'hybride pas dans les conditions habituelles avec les acides nucléiques de campylobactéries n'appartenant pas à cette espèce, ni avec les acides nucléiques génomiques de bactéries proches du genre Campylobacter. Des fragments de cette séquence peuvent être utilisés comme amorces spécifiques pour l'amplification de séquences spécifiques de Campylobacter coli et comme sondes nucléiques spécifiques de séquences nucléiques de Campylobacter coli. L'invention a également pour objet une méthode de détection de la présence de souches de Campylobacter coli dans un échantillon biologique, ainsi qu'un kit pour la mise en oeuvre de la méthode.

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