Nucleotide sequences which hybridize specifically with a...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2132985

The subject of the present invention is thus a nucleotide sequence which hybridizes specifically with a Campylobacter jejuni genomic nucleic acid sequence chosen from the nucleotide sequence SEQ ID No. 1, the nucleotide sequence SEQ ID No. 2 the sequences complementary to the latter, as well as the sequences differing therefrom by nutation, insertion, deletion or substitution of one or more bases, and which does not or virtually does not hybridize with nucleic acids from other Campylobacter species. Fragments of this sequence can be used as specific primers for the amplification of sequences specific for Campylobacter jejuni and as nucleic probes specific for Campylobacter jejuni nucleic sequences. The subject of the invention is also a method of detecting the presence of Campylobacter jejuni stains in a biological sample, as well a kit for carrying out the method.

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