Nucleotide-sugar-synthesizing enzymes from nonparasitic...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/12 (2006.01) C12P 19/30 (2006.01)

Patent

CA 2199093

The present invention relates to nucleotide-sugar-synthesizing enzymes (enzymes with nucleotidyltransferase or pyrophosphorylase activity) from nonparasitic protists, to a process for the preparation thereof and to the use thereof for preparing nucleotide-sugars. The enzymes according to the invention make possible or greatly simplify the enzymatic preparation of various nucleotide-sugars on the industrial scale from low-cost precursors. It is possible with the aid of the discovered enzymes to prepare, for example, GDP-fucose, GDP-mannose, UDP-glucose, UDP-glucosamine, UDP-galactose, UDP-galactosamine, UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in economic quantities.

Enzymes de synthèse de nucléotide-sucres (enzymes possédant une activité de nucléotidyltransférase ou de pyrophosphorylase) provenant de protistes non parasitaires; méthode pour les préparer et leur utilisation pour l'obtention de nucléotide-sucres. Les enzymes de l'invention rendent possible ou simplifient grandement la préparation enzymatique à l'échelle industrielle de divers nucléotide-sucres à partir de précurseurs peu coûteux. Grâce à ces enzymes on peut par exemple préparer les nucléotide-sucres suivants en quantités économiquement rentables : GDP-fucose, GDP-mannose, UDP-glucose, UDP-glucosamine, UDP-galactose, UDP-galactosamine, UDP-N-acétylglucosamine et UDP-N-acétylgalactosamine.

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