Nucleotides labelled with an infrared dye and their use in...

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01) C07F 9/572 (2006.01) C07H 19/04 (2006.01) C07H 19/10 (2006.01) C07H 19/20 (2006.01) C07H 21/00 (2006.01) C09B 23/12 (2006.01)

Patent

CA 2145405

Nucleoside-5'-triphosphates and phosphoramidites which carry a residue absorbing in the long wavelength region, preferably a carbocyanine group of the general formula (I), on the base portion or on the phosphorus atom (see formula I) in which R1 and R2 each denote hydrogen or together form a phenyl residue; R3 denotes hydrogen if linkage with the nucleotide is via the R4 position or it denotes a -NHCS- group if linkage with the nucleotide is via the R3 position; both R4 and R5, or R5, alone denote an alkylsulfonyl group with n being a number from 3 to 5 or R4 represents a -NHCS- group with n being a number from 3 to 8, as well as the use of the compounds to label, detect and sequence nucleic acids.

Nucléoside-5'-triphosphates et phosphoramidites portant, sur la partie de base ou l'atome de phosphore, un reste absorbant dans le domaine des ondes longues, de préférence un groupe carbocyanine de formule générale (I), dans laquelle R<1> et R<2> désignent chacun hydrogène ou forment ensemble un reste phényle; R<3> désigne l'hydrogène, dans le cas où la liaison avec le nucléotide s'effectue par la position R<4>, ou désigne un groupe -NHCS- dans le cas où la liaison avec le nucléotide s'effectue par la position R<3>; R<4> et R<5> désignent chacun, ou R<5> désigne seul, un groupe alkylsulfonyle avec n représentant un nombre compris entre 3 et 5, ou R<4> désigne un groupe -NHCS- avec un nombre de 3 à 8. L'invention concerne également l'utilisation des composés pour le marquage, la détection et le séquençage d'acides nucléiques.

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