C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/04 (2006.01)
Patent
CA 2414485
The invention relates to the field of microbiology, more particularly to a nutritional mixture and to a method for early identification and count of gram- negative microorganisms. Five different colors, three-colored fluorescent emissions, three-colored halos and opaque precipitation zones around the colonies appear in the culture medium depending on the microorganism in question. This makes it possible to establish differentiation with a high degree of sensitivity and specificity. The mixture comprises specific ratios of protein fractions that are rich in free or combined tryptophan, organic and/or inorganic salts, substances that provide color or fluorescence, growth inhibitors of gram-positive organisms, in addition to cellulose and hemicellulose and other components which provide the solid structure of the culture medium.
La présente invention concerne le domaine de la microbiologie et plus particulièrement un mélange nutritif et un procédé d'identification et de dénombrement rapide de micro-organismes Gram-négatifs. Cinq couleurs différentes, des émissions fluorescentes de trois couleurs, des halos de trois couleurs et des zones de précipitation opaque autour des colonies apparaissent dans le milieu de culture en fonction du micro-organisme traité, ce qui permet une différenciation à sensibilité et spécificité élevées. Le mélange comprend des rapports spécifiques de mélanges de fractions protéiques riches en triptophane libre ou combiné, de sels organiques et/ou inorganiques, de substances colorantes ou fluorescentes, d'inhibiteurs de croissance des micro-organismes Gram-positifs, ainsi que de cellulose et d'hémicellulose et d'autres composants conférant une structure solide au milieu de culture.
Quesada Muniz Vivian de Jesus
Rodriguez Martinez Claudio
Tsoraeva Anna
Centro Nacional de Biopreparados
Macrae & Co.
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