C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
150/15, 150/16.4
C12N 1/00 (2006.01) C12Q 1/34 (2006.01)
Patent
CA 1045531
ABSTRACT A diagnostic test system for detecting .beta.-galactosidase producing micro-organisms is prepared by impregnating one æone of the bibulous material with an aqueous acetone solution of an o-nitro-phenyl-.beta.-D-galactopyranoside substrate; and impregnating a contiguous zone of the bibulous material with a solution of a micro-biologically inert buffer system which will maintain a pH of from about 6.5 to 8.5. Preferably, the bibulous material contains 1.5 mg of dry o-nitrophenyl-.beta.-D-galactopyranoside and 0.02 millimoles of a buffer which will maintain a pH of 7.4. Optionally, a waterproof barrier zone and an identifying dye zone may be present. In use, the impregnated bibulous mater- ial is inserted into a test tube of a saline suspension of unknown organism and incubated for four hours. The development of a yellow coloration indi- cates that .beta.-galactosidase is produced by the organism being tested. The diagnostic test system of this invention is stable for one year at 4°C,, at room temperature, and at 37°C.
236377
Kronish Donald P.
Young William D. (jr.)
LandOfFree
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