Octopine t-dna promoters

Details Octopine t-dna promoters Octopine t-dna promoters

C - Chemistry, Metallurgy

12

N

195/1.18, 195/1.

C12N 15/84 (2006.01) C12N 1/21 (2006.01) C12N 15/29 (2006.01) C12N 15/32 (2006.01) C12N 15/82 (2006.01)

Patent

CA 1340764

The sequence of the T-DNA of the octopine-type Ti plasmid found in Agrobacterium tumefaciens ATCC 15955 is disclosed. Fourteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and poly-adenylation sites were found. The use of promoters and polyadenylation sites from pTi15955 to control expression of foreign structural genes is taught, using as examples the structural genes for the Phaseolus vulgaris storage protein phaseolin, P. vulgaris lectin, thaumatin, and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.

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