Ola-based methods for the detection of target nucleic acid...

Details Ola-based methods for the detection of target nucleic acid... Ola-based methods for the detection of target nucleic acid...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2537134

Method for the detection of a target sequence comprising ligating two probes when annealed adjacent to the target sequence, hybridisation of a compound primer to the ligated probes and after elongation of the compound primer, amplifying the elongated compound primer from primers annealing to primer binding sites provided in the compound primer and one of the probes to produce detectably amplicons.

L'invention concerne un procédé permettant de détecter une séquence cible, qui consiste à ligaturer deux sondes lorsqu'elles sont annelées adjacentes à la séquence cible, à hybrider une amorce de composé avec les sondes annelées et après élongation de l'amorce de composé, à amplifier l'amorce de composé allongée à partir d'amorces capables d'annelage avec des sites de liaison d'amorces prévus dans l'amorce de composé et l'une des sondes afin de produire des amplicons de manière détectable.

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