Oligo (alpha-arabinofuranosyl nucleotides) and...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C07H 19/04 (2006.01) C07H 19/06 (2006.01) C07H 19/10 (2006.01) C07H 19/16 (2006.01) C07H 19/20 (2006.01) C07H 21/00 (2006.01) C07H 23/00 (2006.01)

Patent

CA 2089668

ABSTRACT Novel oligonucleotides formed from .alpha.-D-arabinofuranosyl nucleoside monomers, including oligonucleotides in which one or more of the monomer units is functionalized, are disclosed herein, as well as functionalized monomeric .alpha.-D-arabinofuranosyl nucleosides and nucleotides. A generic formula for the oligomers is (Ib), in which B is a nucleotide base which will vary from one monomeric unit to the next in a preselected oligonucleotide sequence; R is phosphate, phosphorothioate, phosphoramidate, or alkanephosphonate; t is 1 for functionalized monomeric units and zero for the others; W is a chemical linker arm; A is a functional group; and n is the number of monomeric units in the oligomer. The oligomers are useful for diagnostic and chemotherapeutic uses. A novel reaction is also disclosed, in which an.alpha.-D-arabinofuranosyl nucleoside with exposed hydroxyls at the 2'-and 3'-positions is selectively protected at the 2'-position in a single reaction.

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