Oligonucleotide-enzyme conjugate and a method for its...

Details Oligonucleotide-enzyme conjugate and a method for its... Oligonucleotide-enzyme conjugate and a method for its...

C - Chemistry, Metallurgy

07

H

195/1.12

C07H 21/00 (2006.01) C12N 11/02 (2006.01)

Patent

CA 2024777

-0- AN OLIGONUCLEOTIDE-ENZYME CONJUGATE AND A METHOD FOR ITS PREPARATION Abstract of the Disclosure A method is provided for preparing a covalent conjugate of an oligonucleotide and an enzyme, such as peroxidase. This conjugate can be used as a probe in hybridization assays and in polymerase chain reaction procedures. The method generally comprises the steps of: reacting an enzyme having a reactive amino group with a mercapto-substituted organic compound to form a blocked intermediate, removing the blocking group to form an enzyme reagent, and reacting the enzyme reagent with a functionalized oligonucleotide reagent.

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