C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/00 (2006.01) C12Q 1/10 (2006.01)
Patent
CA 2286512
Oligonucleotide primers were designed for the PCR-based detection of rfe-rff genes involved in the biosynthesis pathway leading to the production of enterobacterial common antigen (ECA). E. coli DNA was detected, using specific rfe (WecA) and rffT (WecF) gene primers. Moreover, rffT primers allowed the detection of most members of the Enterobacteriaceae family in biological fluids such as blood and urine with sensitivity as low as 120 bacteria per ml of water. Thus, these primers represent an important step in the molecular diagnosis of major Enterobacteriaceae infections and routine testing of contamination in drinking water and food. The invention relates to particular oligonucleotides, the corresponding primers, the use of these primers for PCR detection of Enterobacteriaceae species, the related method for PCR detection and the related diagnostic assay.
Corporation Du Centre de Recherche Du Centre Hospitalier de L'un
Robic
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