Oligonucleotide primers that destabilize non-specific duplex...

Details Oligonucleotide primers that destabilize non-specific duplex... Oligonucleotide primers that destabilize non-specific duplex...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2246623

The present invention relates to genetic engineering. More specifically, a method is presented for reducing mispriming during DNA synthesis. In particular, the present invention relates to universal primers which reduce mispriming during cDNA library construction, thereby increasing the proportion of cDNA clones having been primed from the bona fide 3' poly A tail. The present invention further relates to the use of the discriminating oligonucleotides of the present invention in other methods such as mRNA purification, PCR-based detection methods and sequencing.

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