C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01)
Patent
CA 2324990
Oligonucleotide probes for detecting Enterobacteriaceae species. Unique gyrA coding regions permit the development of probes specific for eight different species: Escherichia coli, Citrobacter freundii, Enterobacter aerogenes, Enterobacter cloacae, Klebsiella oxytoca, Klebsiella pneumoniae, Providencia stuartii and Serratia marcescens. The invention thereby provides methods for the species-specific identification of these Enterobacteriaceae in a sample, and detection and diagnosis of Enterobacteriaceae infection in a subject. Further, nucleic acids are provided for determining quinolone-resistant status of these Enterobacteriaceae.
L'invention concerne des sondes d'oligonucléotides de détection d'espèces d'Enterobacteriaceae. Des régions de codage de gyrA uniques permettent le développement de sondes spécifiques pour huit espèces différentes: Escherichia coli, Citrobacter freundii, Enterobacter aerogenes, Enterobacter cloacae, Klebsiella oxytoca, Klebsiella pneumoniae, Providencia stuartii et Serratia marcescens. L'invention concerne donc des méthodes d'identification spécifique à l'espèce de ces Enterobacteriaceae dans un échantillon et de détection et de diagnostic d'infections dues à Enterobacteriaceae chez un sujet. En outre, l'invention concerne également des acides nucléiques de détermination de l'état de résistance aux quinolones de ces Enterobacteriaceae.
Tenover Fred C.
Weigel Linda M.
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Centers For Disease Co The Government Of The United States Of America As Represented By
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