Oligonucleotide sequence formula for labeling...

C - Chemistry – Metallurgy – 07 – H

Patent

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C07H 21/04 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01) C40B 30/04 (2006.01) C40B 40/06 (2006.01) C40B 70/00 (2006.01) C12N 15/13 (2006.01)

Patent

CA 2703141

The present invention provides oligonucleotide probes and oligonucleotide probe collections and protein labeling for detecting or localizing a plurality nucleic acid target genes or antigens within a cell or tissue sample. Specifically, the provides collections of oligonucleotide probe for use in in situ hybridization analyses in which each probe has a label-domain with the sequence formulas of (CTATTTT)n, (AAAATAG)n or (TTTTATC)n or (GATAAAA)n in which all cases "n" would equal 1 or greater. The present invention provides collections or "cocktails" of oligonucleotide probes for detecting or localizing specific nucleic acid target genes within a cell or tissue sample. The cocktails are useful for detecting the following: the Kappa gene (SEQ ID NOS: 1-16 inclusive); the Lamba gene (SEQ ID NOS: 501-509, 511-513, and 515); the CMV (cytomegalovirus) gene (SEQ ID NOS: 221-241 inclusive); EBER(Epstein-Barr RNA) gene (SEQ ID NOS: 51-54 inclusive): Alu(SEQ IN NOS: 55-56); PolyA(SEQ ID NO: 57); and the detection tail (SEQ ID NO: 330).

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