Oligonucleotide tags for sorting and identification

Details Oligonucleotide tags for sorting and identification Oligonucleotide tags for sorting and identification

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2332731

The invention provides a method of tracking, identifying and/or sorting classes or subpopulation of molecules by the use of oligonucleotide tags. Oligonucleotide tags of the invention comprise oligonucleotides selected from a minimally cross-hybridizing set. Preferably, such oligonucleotides each consist of a plurality of subunits 3 to 9 nucleotides in length. A subunit of a minimally cross- hybridizing set forms a duplex or triplex having two or more mismatches with the complement of any other subunit of the same set. The number of oligonucleotide tags available in a particular embodiment depends on the number of subunits per tag and on the length of the subunit. An important aspect of the invention is the use of the oligonucleotide tags for sorting polynucleotides by specifically hybridizing tags attached to the polynucleotides to their complements on solid phase supports. This embodiment provides a readily automated system for manipulating and sorting polynucleotides, particularly useful in large-scale parallel operations, such as large-scale DNA sequencing, mRNA fingerprinting, and the like, wherein many target polynucleotides or many segments of a single target polynucleotide are sequenced simultaneously.

Affiliated with

Also associated with

Rating

Oligonucleotide tags for sorting and identification does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Oligonucleotide tags for sorting and identification, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Oligonucleotide tags for sorting and identification will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1926754