C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/08 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2108247
The use of the superoxide dismutase (SOD; EC 1.15.1.1) gene system as a target for the detection and differentiation of pathogenic and non-pathogenic organisms including bacteria, fungi and protozoans is described. More particularly, there are described oligonucleotides derived from the SOD gene family which oligonucleotides are capable of hybridizing to a single-stranded nucleic acid sequence (target sequence) which is obtainable by amplifying and maturing a part of a gene coding for a superoxide dismutase (SOD) using a polymerase chain reaction (PCR) and a pair of primers, the first primer containing the sequence 5'-AGC TTC ACC ACA GCA AGC ACC A-3' (Seq. Id. No. 1; Z205) as priming sequence and the second primer containing the sequence 5'-TCG G(T)CC CAG TTC ACG ACG(A,T) TTC CA-3' (Seq. Id. No. 2; Z212) as priming sequence. These oligonucleotides in the form of primers and probes are useful for the amplification and detection of target sequences derived from SOD genes, especially, from SOD genes of pathogenic and non-pathogenic organisms including bacteria (e.g. mycobacteria), fungi and protozoans.
Borden Ladner Gervais Llp
F. Hoffmann-La Roche Ag
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