Oligonucleotides for the amplification and detection of...

Details Oligonucleotides for the amplification and detection of... Oligonucleotides for the amplification and detection of...

C - Chemistry, Metallurgy

12

Q

C12Q 1/70 (2006.01)

Patent

CA 2321654

The present invention is concerned with oligonucleotides that can be used as in the amplification and detection of Epstein Barr Virus (EBV) nucleic acid, in particular RNA-specific sequences. Furthermore a method for the diagnosis of EBV associated malignant and non-malignant diseases is provided. The oligonucleotides according to the present invention are specifically suited for the detection of EBV gene expression in circulating peripheral blood cells, in human (tumor) tissue samples and thin sections thereof using "in solution" amplification or "in situ" amplification techniques and in other biological samples potentially containing EBV-infected cells.

La présente invention concerne des oligonucléotides pouvant être utilisés dans l'amplification et la détection de l'acide nucléique du virus Epstein-Barr, en particulier de séquences spécifiques de l'ARN. L'invention se rapporte également à un procédé de diagnostic d'affections malignes et bénignes associées au virus Epstein-Barr. Les oligonucléotides de la présente invention sont particulièrement adaptés à la détection de l'expression génique du virus Epstein-Barr dans les cellules sanguines périphériques circulantes, dans des prélèvements de tissus humains (tumoraux) et de minces sections de ces derniers via des techniques d'amplification "en solution" ou d'amplification "in situ", ainsi que dans d'autres prélèvements biologiques susceptibles de contenir des cellules infectées par le virus Epstein-Barr.

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