Oligonucleotides having a-dna form and b-dna form...

C - Chemistry – Metallurgy – 07 – H

Patent

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C07H 21/02 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2373225

Modified oligonucleotides containing both A-form conformation geometry and B- form conformation geometry nucleotides are disclosed. The B-form geometry allows the oligonucleotide to serve as substrates for RNase H when bound to a target nucleic acid strand. The A-form geometry imparts properties to the oligonucleotide that modulate binding affinity and nuclease resistance. By utilizing C2' endo sugars or O4' endo sugars, the B-form characteristics are imparted to a portion of the oligonucleotide. The A-form characteristics are imparted via use of either 2'-O-modified nucleotides that have 3' endo geometries or use of end caps having particular nuclease stability or by use of both of these in conjunction with each other.

La présente invention concerne des oligonucléotides modifiés contenant des nucléotides à la fois de géométrie de conformation à la forme A et de géométrie de conformation à la forme B. La géométrie de conformation à la forme B permet au nucléotide de servir de substrat pour RNase H lorsqu'il est lié à un brin d'acide nucléique cible. La géométrie de forme A transmet des propriétés au nucléotide qui modulent l'affinité de liaison et la résistance à la nucléase. En utilisant C2' endo-sucres ou O4' endo-sucres, les caractéristiques de la forme B sont transmis à une partie de l'oligonucléotide. Les caractéristiques de la forme A sont transmis par 2'-O-nucléotides modifiés présentant 3' géométries endo, ou par des coiffes d'extrémité présentant une stabilité particulière à la nucléase, ou par ces deux possibilités simultanément.

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