Oligonucleotides having phosphorothioate linkages of high...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) A61K 31/70 (2006.01) C07H 21/00 (2006.01)

Patent

CA 2223103

Sequence-specific phosphorothioate oligonucleotides comprising nucleoside units which are joined together by either substantially all Sp or substantially all Rp phosphorothioate intersugar linkages are provided. Such sequence-specific phosphorothioate oligonucleotides having substantially chirally pure intersugar linkages are prepared by enzymatic or chemical synthesis.

Des oligonucléotides ayant une séquence spécifique sont constitués d'unités nucléosidiques liées par des liaisons phosphorothioate entre les oses qui ont toutes sensiblement la configuration Sp ou la configuration Rp. Ces séquences spécifiques d'oligonucléotides qui ont des liaisons phosphorothioate entre les oses et qui sont sensiblement pures du point de vue chiral sont préparées par synthèse enzymatique ou chimique.

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