Oncofetal fibronectin epitope

Details Oncofetal fibronectin epitope Oncofetal fibronectin epitope

C - Chemistry, Metallurgy

07

K

530/7.06

C07K 7/06 (2006.01)

Patent

CA 1339622

An onco-developmentally regulated .alpha.-N-acetyl- galactosaminyltransferase isolated as a component of a particulate membrane fraction separated from cell and tissue homogenates and having the following characteristics: (a) Activity in Various Cells and Tissues -- present in human fetal lung fibroblasts, hepatoma tissues and placenta, but absent from normal adult liver, lung, kidney and spleen tissues; (b) Substrate Specificity -- acts on polypeptides comprising a sequence val-thr-his-pro-gly-tyr by catalyzing .alpha.-N-acetylgalactosaminylation at the thr residue of the sequence; (c) Requirements for Metal Ion -- requires metal ion for activity in 25 mM tris buffer, pH 7.6; (d) Optimal pH -- optimal pH is about 7.6 assayed in hepatoma cell homogenate in tris buffer and at a pH of about 6 to about 7 assayed in hepatoma cell homogenate; enzyme activity is higher in 2(N-morpholino)ethanesulfonic acid than in cacodylate buffer; and (e) Km -- apparent Km for UDP - GalNAc is about 48 µM.

617045

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