C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/97, 195/33.1
C12N 15/55 (2006.01) C12N 9/80 (2006.01) C12N 9/86 (2006.01) C12P 35/02 (2006.01) C12P 35/06 (2006.01)
Patent
CA 2019625
17964 TITLE OF THE INVENTION A ONE-STEP CEPHALOSPORIN C AMIDASE ENZYME, A GENE ENCODING THE SAME, AND EXPRESSION THEREOF IN A SUITABLE HOST ABSTRACT OF THE DISCLOSURE A process for the one-step conversion of cephalosporin C and derivatives thereof to the corresponding 7-aminocephalosporanic acid and derivatives comprising treating said cephalosporin C and derivatives with a cephalosporin C amidase enzyme of a recited sequence, the DNA encoding said enzyme, and expression thereof in a suitable host, e.g., Bacillus species under the control of a suitable promoter.
Crawford Mark S.
Finkelstein David B.
Rambosek John A.
Crawford Mark S.
Finkelstein David B.
Merck & Co. Inc.
Rambosek John A.
Swabey Ogilvy Renault
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