One-step reduction and alkylation of proteins

Details One-step reduction and alkylation of proteins One-step reduction and alkylation of proteins

C - Chemistry, Metallurgy

07

K

C07K 1/113 (2006.01)

Patent

CA 2537701

The present invention relates generally to the field of analyzing proteins and more specifically to the reduction and alkylation of protein samples during protein analysis. A novel composition and method for reducing and alkylating proteins is disclosed. A novel reagent including a combination of a volatile reducing agent, a volatile alkylating agent, and a volatile solvent is used for a one~step reduction and alkylation of proteins allowing the protein sample to remain in the same container during the reduction and alkylation processes.

La présente invention se rapporte généralement au domaine de l'analyse des protéines, et plus particulièrement à celui de la réduction et de l'alkylation d'échantillons de protéines pendant l'analyse des protéines. On décrit une nouvelle composition et une méthode de réduction et d'alkylation de protéines. Un nouveau réactif comprenant une combinaison d'un agent réducteur volatil, d'un agent d'alkylation volatil et d'un solvant volatil est utilisé pour une réduction et une alkylation de protéines en une étape qui permettent à l'échantillon de protéines de rester dans le même contenant au cours des processus de réduction et d'alkylation.

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