Opsonic and protective monoclonal and chimeric antibodies...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 16/00 (2006.01) C07K 14/195 (2006.01) C07K 16/12 (2006.01) A61K 38/00 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2293732

The present invention encompasses monoclonal and chimeric antibodies that bind to lipoteichoic acid of Gram positive bacteria. The antibodies also bind to whole bacteria and enhance phagocytosis and killing of the bacteria in vitro and enhance protection from lethal infection in vivo. The mouse monoclonal antibody has been humanized and the resulting chimeric antibody provides a previously unknown means to diagnose, prevent and/or treat infections caused by gram positive bacteria bearing lipoteichoic acid. This invention also encompasses a peptide mimic of the lipoteichoic acid epitope binding site defined by the monoclonal antibody. This epitope or epitope peptide mimic identifies other antibodies that may bind to the lipoteichoic acid epitope. Moreover, the epitope or epitope peptide mimic provides a valuable substrate for the generation of vaccines or other therapeutics.

La présente invention concerne des anticorps monoclonaux et chimères qui se fixent à l'acide lipotéichoïque des bactéries Gram positif. Ces anticorps, qui se fixent également à toutes ces bactéries, favorisent la phagocytose et l'élimination de ces bactéries in vitro, tout en améliorant la protection contre toute infection mortelle in vivo. L'anticorps monoclonal de la souris a été humanisé, l'anticorps chimère ainsi obtenu constituant un moyen jusqu'alors inconnu de diagnostic, de prévention et/ou de traitement des infections provoquées par les bactéries Gram positif porteuses d'acide lipotéichoïque. Cette invention concerne également un analogue peptidique du site de fixation à l'épitope acide défini par ledit anticorps monoclonal. Cet épitope ou cet analogue peptidique de l'épitope identifie d'autres anticorps pouvant se fixer à l'épitope de l'acide lipotéichoïque. En outre, cet épitope ou cet analogue peptidique de l'épitope constitue un substrat adapté à la production de vaccins ou d'autres moyens thérapeutiques.

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