Optical biosensor

Details Optical biosensor Optical biosensor

G - Physics

01

N

150/26, 73/51.5

G01N 33/52 (2006.01) C07D 277/32 (2006.01) C07D 277/56 (2006.01) C07D 311/16 (2006.01) C07D 417/04 (2006.01) G01N 21/64 (2006.01) G01N 21/76 (2006.01) G01N 33/533 (2006.01) G01N 33/542 (2006.01) G01N 33/543 (2006.01) G01N 33/552 (2006.01) G01N 33/553 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2030244

Optical Biosensor A b s t r a c t A new optical biosensor based on fluorescence energy transfer, consisting of a) a solid support, b) a single-layer or multilayer Langmuir-Blodgett (LB) film attached to a), c) at least one fluorescent dye F1 which is located in at least one of the top 4 layers of the LB film, d) a molecule (receptor) which is capable of specific interaction and which is bound or located in or on the topmost layer of the LB film, and e) a mobile fluorescent dye F2 whose excitation band overlaps, sufficiently for an energy transfer, with the emission band F1 and which e1) is covalently bonded to a ligand which is able to bind to the receptor, or which e2) is covalently bonded to another receptor which is able to bind to the complex composed of the first receptor and ligand, where the ligand or the ligand and the second receptor are initially not bound to the LB film, is described. Le A 27 335US

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