Optical fiber delivery and collection system for biological...

A - Human Necessities – 61 – B

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A61B 1/07 (2006.01) A61B 1/04 (2006.01) A61B 10/04 (2006.01) G01N 21/63 (2006.01) G01N 21/64 (2006.01) G02B 21/06 (2006.01)

Patent

CA 2535843

The present invention is directed to a method of applying radiation through an optical fiber for detecting disease within a plant or animal or other penetrable tissue, or imaging a particular tissue of a plant or animal. In addition, fluorescence an nonlinear scattering signals can be detected and localized within a subject by such application of radiation through an optical fiber. The radiation is effective to promote simultaneous multiphoton excitation. The optical fibers are used alone to examine internal regions of tissue, in conjunction with an optical biopsy needle to evaluate sub-surface tissue, or with an endoscope to evaluate tissue within body cavities. The present invention also relates to a device for coupling in radiation from an ultrashort mode-locked laser into the beam path of a microscope.

La présente invention concerne un procédé permettant d'appliquer un rayonnement dans une fibre optique pour la détection d'une maladie dans une plante ou chez un animal ou autre tissu pénétrable, ou pour l'imagerie d'un tissu particulier d'une plante ou d'un animal. Une telle application de rayonnement dans une fibre optique permet également de détecter et de localiser des signaux de diffusion non linéaire et de fluorescence chez un sujet. Le rayonnement favorise efficacement une excitation multiphotonique simultanée. Les fibres optiques sont utilisées seules afin d'examiner des zones internes de tissus ; elles sont utilisées avec une aiguille de biopsie optique afin d'évaluer un tissu sub-superficiel ou avec un endoscope afin d'évaluer le tissu dans les cavités de l'organisme. La présente invention concerne également un dispositif permettant de coupler un rayonnement d'un laser verrouillé en mode ultracourt dans la voie du faisceau d'un microscope.

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