Optical sensor system for the determination of ph values...

C - Chemistry – Metallurgy – 08 – F

Patent

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C08F 220/56 (2006.01) G01N 21/64 (2006.01)

Patent

CA 2235343

The invention relates to a polymer composition comprising pH-sensitive fluorescent dyes, to an ionic strength-independent optical sensor for pH value determination that contains the composition in the form of a membrane on a transparent support material, and to an optical process, according to the fluorescence method, that renders possible highly accurate pH value determination independently of the ionic strength of the test solution. The process is especially suitable for the determination of the pH value of physiological solutions, especially for the determination of the pH value of blood. The water-insoluble copolymer is composed of: a) from 39.9 to 60 % by weight of N,N-dimethylacrylamide or N,N-dimethylmethacrylamide; b) from to 60 to 39.9 % by weight of a monomer of formula (Ia) or (Ib) wherein Ra is hydrogen or C1-C6alkyl and Rb is C1-C12alkyl; c) from 0.1 to 0.7 % by weight of a proton-sensitive fluorophore which is either admixed with or covalently bonded to the polymer; and d) from 0 to 20 % by weight of a diolefinic crosslinking component, the sum of the percentage weights of a) to d) being 100 %.

L'invention se rapporte à une composition polymère comprenant des colorants fluorescents sensibles au pH, ainsi qu'un capteur optique, lequel est indépendant de la force ionique, est destiné à déterminer des valeurs de pH et contient la composition sous la forme d'une membrane placée sur un matériau support transparent. L'invention concerne encore un procédé conforme au système de fluorescence et permettant de déterminer des valeurs de pH extrêmement précises, indépendamment de la force ionique de la solution d'essai. Ce procédé est notamment approprié à la détermination de la valeur du pH de solutions physiologiques, particulièrement à la détermination de la valeur du pH du sang. Le copolymère insoluble dans l'eau se compose en poids: a) de 39,9 à 60 % de N,N-diméthylacrylamide ou de N,N-diméthylméthacrylamide; b) de 60 à 39,9 % d'un monomère de la formule (Ia) ou de la formule (Ib), dans lesquelles R¿a? représente hydrogène ou alkyle C¿1?-C¿6? et R¿b? représente alkyle C¿1?-C¿12?; c) de 0,1 à 0,7 % d'un fluorophore sensible aux protons, lequel est soit mélangé au polymère, soit lié de manière covalente à celui-ci; et d) de 0 à 20 % d'un agent de réticulation dioléfinique, la somme des pourcentages pondéraux de a) à d) étant de 100 %.

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