Optical sensors based on hybrid aptamer/conjugated polymer...

Details Optical sensors based on hybrid aptamer/conjugated polymer... Optical sensors based on hybrid aptamer/conjugated polymer...

G - Physics

01

N

G01N 33/52 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2430910

A new selective, rapid and highly sensitive method (as few as 2 × 10 -15 mole) for the detection of human .alpha.-thrombin is disclosed, based on the formation of hybrid anionic aptamer/cationic polythiophene complexes. Advantageously, this new method does not require any chemical reaction on the probes or the analytes and is based on conformational modifications of the conjugated backbone of a cationic poly(3-alkoxy-4-methylthiophene), when mixed with ss-DNA and a target protein.

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