Optical sorting method

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2357037

The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; (b) expressing the genetic elements to produce their respective gene products within the microcapsules; (c) sorting the genetic elements which produce the gene product having the desired activity using a change in the optical properties of the genetic elements. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.

L'invention concerne une technique permettant d'isoler un ou plusieurs éléments génétiques qui codent pour un produit génique possédant une activité désirée. La technique comprend les étapes suivantes: (a) on compartimente les éléments génétiques de façon à les répartir dans plusieurs microcapsules; (b) on induit l'expression des différents éléments génétiques afin qu'ils produisent leurs produits géniques respectifs à l'intérieur des microcapsules; (c) on trie les éléments génétiques qui produisent le produit génique possédant l'activité désirée en se basant sur une modification des propriétés optiques desdits éléments. L'invention permet l'évolution in vitro des acides nucléiques et des protéines par mutagenèses répétées et applications itératives de la technique ci-dessus.

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