Optimally fluorescent oligonucleotides

Details Optimally fluorescent oligonucleotides Optimally fluorescent oligonucleotides

C - Chemistry, Metallurgy

12

P

C12P 19/34 (2006.01) C07H 21/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2229017

A method for the preparation of optimally fluorescent oligonucleotides wherein fluorescent dye-conjugated nucleotide triphosphates are incorporated into a nucleic acid sequence in a defined repetitive manner which allows for the optimal specific fluorescence of the oligonucleotide. The oligonucleotides of the present invention are useful in the assay of a wide variety of nucleic acid sequences, specifically wherever highly fluorescent nucleic acid probes are desired.

L'invention concerne un procédé de préparation d'oligonucléotides à fluorescence optimale, procédé au cours duquel des triphosphates nucléotidiques conjugués à des colorants fluorescents sont incorporés dans une séquence d'acides nucléiques de manière définie et répétitive pour permettre une fluorescence spécifique optimale de l'oligonucléotide. Ces oligonucléotides sont utilisés dans l'analyse d'une grande variété de séquences d'acides nucléiques, spécifiquement partout où sont souhaitées des sondes d'acide nucléique à fluorescence optimale.

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