Optimization of excitation voltage amplitude for collision...

H - Electricity – 01 – J

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H01J 49/42 (2006.01)

Patent

CA 2716645

Collision induced dissociation of precursor ions in an ion trap is performed by determining a predicted fragmentation-optimized excitation voltage amplitude based on an indicator of damping gas pressure, such as a damping gas flow rate, and optionally other parameters including precursor ion m/z and an indicator of the Mathieu parameter q. The excitation voltage may then be applied to electrodes of the ion trap in steps of increasing amplitude, wherein at least one of the amplitudes corresponds to the predicted optimum value. Application of the excitation voltage in this manner produces favorable fragmentation efficiencies over a range of operating parameters and for ions of differing chemical properties.

La présente invention concerne une dissociation dions précurseurs induite par collision dans un piège à ions qui est réalisée par la détermination dune amplitude de tension dexcitation optimisée par fragmentation prédite sur la base dun indicateur dune pression gazeuse damortissement, tel quun débit gazeux damortissement, et facultativement dautres paramètres comprenant lion précurseur m/z et un indicateur du paramètre de Mathieu q. La tension dexcitation peut ensuite être appliquée aux électrodes du piège à ions dans des étapes consistant à augmenter lamplitude, au moins lune des amplitudes correspondant à la valeur optimum prédite. Lapplication de la tension dexcitation de cette manière produit des efficacités de fragmentation favorables sur une plage de paramètres de fonctionnement et pour des ions ayant des propriétés chimiques différentes.

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