Optimization of pest resistance genes using dna shuffling

Details Optimization of pest resistance genes using dna shuffling Optimization of pest resistance genes using dna shuffling

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) C07K 14/325 (2006.01) C12N 5/14 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2325567

This invention provides methods of obtaining pest resistance genes that are improved over naturally occurring genes for use in conferring upon plants resistance to pests. The methods involve the use of DNA shuffling of pest resistance genes to produce libraries of recombinant pest resistance genes, which are then screened to identify those that exhibit the improved property or properties of interest.

La présente invention concerne des méthodes qui permettent d'obtenir des gènes de résistance améliorés par rapport aux gènes naturels et qui rendent les plantes résistantes aux ravageurs. Ces méthodes font intervenir un réarrangment d'ADN chez des gènes de résistance et permettent d'obtenir des librairies de gènes de résistance recombinants dont le criblage fait apparaître ceux d'entre eux qui présentent une propriété ou des propriétés d'intérêt.

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